Ravi Sachidanandam, Cold
Spring Harbor Laboratory, NY.
a splice site database"
We recently built a curated collection of over half a million splice
sites from five species, H. sapiens, M. musculus, D. melanogaster,
C. elegans and A. thaliana. This has allowed us to infer properties
of splice sites and the splicing machinery, that individual
experiments would find hard to infer.
I will take information on splicing from several genomes and use
various numbers (the properties of introns/exons such as categories,
counts and sizes) to dissect the biology. I aim to touch upon various
details in collecting this information and the problems with common
pattern recognition approaches to ab-initio prediction of the
splice-sites. A variety of splicing phenomena will be addressed by the
analyses, such as mechanisms of splice-site recognition, dynamics of
the splicing reaction etc. In addition, the talk will concentrate on
what the numbers say about the evolution of genes and the evolution of
the splicing machinery and conclude with an inflationary model of the